Welcome to Ask Stago, The Podcast dedicated to provide expert answers to your expert questions in coagulation. In today episode, our expert Vicki, will give you all her tips to collect and prepare you sample in the best way.  From sample, material or storage be sure to provide valid sample to your lab.  A podcast to share with your colleague, nurses or any person in charge of the preparation of the sample in your facility.  

As usual, don’t forget to send any question you may have to Ask@stago.com, we will be glad to answer to it.  

To learn more: Continue with our podcast dedicated to the HIL pre-analytical check https://www.podcastics.com/podcast/episode/9-how-to-manage-hil-samples-in-the-coagulation-laboratory-37748/ 

Literature: 

• Stability of coagulation proteins in frozen plasma. B. Woodhams, O. Girardot, MJ Blanco and Y.Gourmelin, Blood coagulation and fibrinolysis 2001, 12:229-236 
• Effects of storage time and temperature on coagulation tests and factors in fresh plasma Limin Feng, Ying Zhao, Hongcan Zhao & Zhexin Shao Scientific Reports 4, Article number: 3868 (2014) doi:10.1038/srep03868 
• Quality Standards for Sample Collection in Coagulation Testing Giuseppe Lippi, M.D. 1 Gian Luca Salvagno, M.D. 2 Martina Montagnana, M.D. 2 Gabriel Lima-Oliveira, M.D. 2 Gian Cesare Guidi, M.D. 2 Emmanuel J. Favaloro, Ph.D., M.A.I.M.S., F.F.Sc. (RCPA) 3 Semin Thromb Hemost 2012;38:565–575. 
• Quality in Laboratory Hemostasis and Thrombosis. S.Kitchen, JD Olson, E.Preston. Wiley-Blackwell 2009 
• Quality Standards for Sample Processing,Transportation, and Storage inHemostasis Testing Dorothy M. Adcock Funk M.D., Giuseppe Lippi M.D., Emmanuel J. Favaloro Ph.D., Semin Thromb Hemost 2012;38:576–585. 
• Pre-analytical Variables in Coagulation Testing Associated With Diagnostic Errors in Hemostasis Emmanuel J. Favaloro, PhD, MAIMS, FFSc (RCPA); Dorothy M. (Adcock) Funk, MD; Giuseppe Lippi, MD DisclosuresLab Med. 2012;43(2):1-10.  
• Effects of haemolysis, icterus and lipaemia on coagulation tests as performed on Stago STA-Compact-Max analyser, A. WOOLLEY, J.-L. GOLMARD, S. KITCHEN, 2016 John Wiley & Sons Ltd, Int. Jnl. Lab. Hem. 
• Recommandation préanalytiques en hémostase; GFHT 2017-2018 
• CLSI Collection, Transport and Processing of Blood Specimens for Testing Plasma-based Coagulation Assays and Molecular Hemostasis Assays; Approved Guideline – Fifth Edition. CLSI Document H21-A5; Wayne, PA: Clinical and Laboratory Standards Institute; 2008 
• Alesci S, Borggrefe M, Dempfle CE. Effect of freezing method and storage at -20⁰C and -70⁰C on prothrombin time, aPTT and plasma fibrinogen levels. Thromb Res. 2009;124(1):121-126. 
• van den Besselaar AM, Witteveen E, van der Meer FJ. Long-term stability of frozen pooled plasmas stored at -70°C, -40°C, and -20°C for prothrombin time and International Normalized Ratio (INR) assessment. Thromb Res. 2013 Apr;131(4):349-51. doi: 10.1016/j.thromres.2013.01.020. Epub 2013 Feb 12. PMID: 23411127. 

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Content is scientific and technical in nature. It is intended as an educational tool for laboratory professionals and topics discussed are not intended as recommendations or as commentary on appropriate clinical practice.